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primary antibodies against cd86 nbp2-25208  (Novus Biologicals)


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    Novus Biologicals primary antibodies against cd86 nbp2-25208
    Effect of CEMMS on the polarization of rat macrophages. A) Quantitative analysis of M1 macrophage-related phenotypes using real-time PCR (n = 3). B) Quantitative analysis of M2 macrophage-related phenotypes using real-time PCR (n = 3). C) Immunofluorescence staining for markers of <t>macrophages(CD86;</t> Green)/(CD206; Red). Scale bars: 100 μm. D) Quantitative analysis of immunofuorescence staining. E) Immunofluorescence staining for markers of macrophages (Arg-1; Green)/(IL-1β; Red). Scale bars: 100 μm. F) Quantitative analysis of immunofuorescence staining. ∗∗∗∗ p < 0.0001.
    Primary Antibodies Against Cd86 Nbp2 25208, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against cd86 nbp2-25208/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
    primary antibodies against cd86 nbp2-25208 - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Construction of cartilaginous organoids based on cartilage extracellular matrix microcarriers to promote articular cartilage regeneration through immune regulation"

    Article Title: Construction of cartilaginous organoids based on cartilage extracellular matrix microcarriers to promote articular cartilage regeneration through immune regulation

    Journal: Journal of Orthopaedic Translation

    doi: 10.1016/j.jot.2025.05.005

    Effect of CEMMS on the polarization of rat macrophages. A) Quantitative analysis of M1 macrophage-related phenotypes using real-time PCR (n = 3). B) Quantitative analysis of M2 macrophage-related phenotypes using real-time PCR (n = 3). C) Immunofluorescence staining for markers of macrophages(CD86; Green)/(CD206; Red). Scale bars: 100 μm. D) Quantitative analysis of immunofuorescence staining. E) Immunofluorescence staining for markers of macrophages (Arg-1; Green)/(IL-1β; Red). Scale bars: 100 μm. F) Quantitative analysis of immunofuorescence staining. ∗∗∗∗ p < 0.0001.
    Figure Legend Snippet: Effect of CEMMS on the polarization of rat macrophages. A) Quantitative analysis of M1 macrophage-related phenotypes using real-time PCR (n = 3). B) Quantitative analysis of M2 macrophage-related phenotypes using real-time PCR (n = 3). C) Immunofluorescence staining for markers of macrophages(CD86; Green)/(CD206; Red). Scale bars: 100 μm. D) Quantitative analysis of immunofuorescence staining. E) Immunofluorescence staining for markers of macrophages (Arg-1; Green)/(IL-1β; Red). Scale bars: 100 μm. F) Quantitative analysis of immunofuorescence staining. ∗∗∗∗ p < 0.0001.

    Techniques Used: Real-time Polymerase Chain Reaction, Immunofluorescence, Staining



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    Effect of CEMMS on the polarization of rat macrophages. A) Quantitative analysis of M1 macrophage-related phenotypes using real-time PCR (n = 3). B) Quantitative analysis of M2 macrophage-related phenotypes using real-time PCR (n = 3). C) Immunofluorescence staining for markers of <t>macrophages(CD86;</t> Green)/(CD206; Red). Scale bars: 100 μm. D) Quantitative analysis of immunofuorescence staining. E) Immunofluorescence staining for markers of macrophages (Arg-1; Green)/(IL-1β; Red). Scale bars: 100 μm. F) Quantitative analysis of immunofuorescence staining. ∗∗∗∗ p < 0.0001.
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    Image Search Results


    Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vivo . (A–C) Elisa was used to examine the expression levels of IL-6, TNF-α and IL-10 expression level. (D–F) Real-time qRT-PCR analysis of the relative mRNA expression of TMEM119 , iNOS , and Arg1 in the DLPFC. (G, H) Immunofluorescence staining of Iba-1, M1-type (CD86) and M2-type (CD206) markers in the left PFC region. (I, J) Proportion of positive cells for target proteins. The number of samples in each group is 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ***P < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

    doi: 10.3389/fimmu.2025.1666920

    Figure Lengend Snippet: Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vivo . (A–C) Elisa was used to examine the expression levels of IL-6, TNF-α and IL-10 expression level. (D–F) Real-time qRT-PCR analysis of the relative mRNA expression of TMEM119 , iNOS , and Arg1 in the DLPFC. (G, H) Immunofluorescence staining of Iba-1, M1-type (CD86) and M2-type (CD206) markers in the left PFC region. (I, J) Proportion of positive cells for target proteins. The number of samples in each group is 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ***P < 0.001.

    Article Snippet: Primary antibodies against CD86 (1:100; bs-1035r; Bioss), CD206 (1:100; #24595; CST) and Iba-1(1:100, ab283319, Abcam, Cambridge, UK)were applied, and the sections were incubated overnight at 4 °C.

    Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Immunofluorescence, Staining

    Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vitro . (A–D) Elisa was used to examine the expression levels of NLRP3, IL-6, TNF-α and IL-10 expression level. (E–G) Immunofluorescence staining of Iba-1, CD86, CD206. (H–J) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ****P < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

    doi: 10.3389/fimmu.2025.1666920

    Figure Lengend Snippet: Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vitro . (A–D) Elisa was used to examine the expression levels of NLRP3, IL-6, TNF-α and IL-10 expression level. (E–G) Immunofluorescence staining of Iba-1, CD86, CD206. (H–J) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ****P < 0.0001.

    Article Snippet: Primary antibodies against CD86 (1:100; bs-1035r; Bioss), CD206 (1:100; #24595; CST) and Iba-1(1:100, ab283319, Abcam, Cambridge, UK)were applied, and the sections were incubated overnight at 4 °C.

    Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Expressing, Immunofluorescence, Staining

    The roles of METTL3 and NMDAR2B in the regulation of microglial cell polarization by magnetic stimulation in vitro . (A–C, G–I) Immunofluorescence staining of Iba-1, CD86 and CD206. (D–F, J–L) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

    doi: 10.3389/fimmu.2025.1666920

    Figure Lengend Snippet: The roles of METTL3 and NMDAR2B in the regulation of microglial cell polarization by magnetic stimulation in vitro . (A–C, G–I) Immunofluorescence staining of Iba-1, CD86 and CD206. (D–F, J–L) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

    Article Snippet: Primary antibodies against CD86 (1:100; bs-1035r; Bioss), CD206 (1:100; #24595; CST) and Iba-1(1:100, ab283319, Abcam, Cambridge, UK)were applied, and the sections were incubated overnight at 4 °C.

    Techniques: In Vitro, Immunofluorescence, Staining

    Effect of CEMMS on the polarization of rat macrophages. A) Quantitative analysis of M1 macrophage-related phenotypes using real-time PCR (n = 3). B) Quantitative analysis of M2 macrophage-related phenotypes using real-time PCR (n = 3). C) Immunofluorescence staining for markers of macrophages(CD86; Green)/(CD206; Red). Scale bars: 100 μm. D) Quantitative analysis of immunofuorescence staining. E) Immunofluorescence staining for markers of macrophages (Arg-1; Green)/(IL-1β; Red). Scale bars: 100 μm. F) Quantitative analysis of immunofuorescence staining. ∗∗∗∗ p < 0.0001.

    Journal: Journal of Orthopaedic Translation

    Article Title: Construction of cartilaginous organoids based on cartilage extracellular matrix microcarriers to promote articular cartilage regeneration through immune regulation

    doi: 10.1016/j.jot.2025.05.005

    Figure Lengend Snippet: Effect of CEMMS on the polarization of rat macrophages. A) Quantitative analysis of M1 macrophage-related phenotypes using real-time PCR (n = 3). B) Quantitative analysis of M2 macrophage-related phenotypes using real-time PCR (n = 3). C) Immunofluorescence staining for markers of macrophages(CD86; Green)/(CD206; Red). Scale bars: 100 μm. D) Quantitative analysis of immunofuorescence staining. E) Immunofluorescence staining for markers of macrophages (Arg-1; Green)/(IL-1β; Red). Scale bars: 100 μm. F) Quantitative analysis of immunofuorescence staining. ∗∗∗∗ p < 0.0001.

    Article Snippet: The cells were incubated with primary antibodies against CD86 (NBP2-25208, Novus), CD206 (24595S, CST), IL-1β(ab254360, Abcam) and Arg-1 (66129-1-Ig, proteintech) overnight at 4 °C.

    Techniques: Real-time Polymerase Chain Reaction, Immunofluorescence, Staining